Dibutyl phthalate influences the secretion of rat ovarian granulose cells

doi:10.3969/j.issn.2095-4344.2014.42.011

Abstract

Abstract:

BACKGROUND: Studies have shown that dibutyl phthalate has certain toxicity to the reproductive system of male animals.
OBJECTIVE: To investigate the effects of dibutyl phthalate on secretion of rat ovarian granulose cells.
METHODS: Ovarian granulosa cells from 25-day-old female Sprague-Dawley rats were cultured in vitro and exposed to different concentrations of dibutyl phthalate (0, 5, 20, 80 μmol/L) for 24 hours. Estradiol and progesterone in ovarian granulosa cells were measured by the method of radio-immunity. Expression of the cytochrome P450aromatase and P450 side chain cleavage enzyme mRNA in granulosa cells were examined by real-time PCR.
RESULTS AND CONCLUSION: The levels of estradiol and progesterone in granulosa cells decreased after exposure to dibutyl phthalate. With the increase of the dibutyl phthalate concentration, the expression of P450arom and P450scc mRNA decreased gradually. Dibutyl phthalate could induce reproductive dysfunction and toxicity to female rats.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: phthalic acids, cytochromes, urogenital system, estradiol, progesterone

CLC Number:

R318

Wang Xue-jin, Zhou Yun-qing, Luo Xiang-min, Liu Jin . Dibutyl phthalate influences the secretion of rat ovarian granulose cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(42): 6779-6783.

Figures/Tables 1

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